For our project we will be using four fish samples (one saltwater, one freshwater and two fresh/salt water) to determine if there are any differences or similarities in their protein size that would have been influenced by the fresh/salt water being able to adapt between two different environments.
According to an article posted by the public library of science salinity is a key factor that affects metabolism, survival and distribution of fish species (Laudet). The study conducted by PLoS ONE investigated the gene expression of regulatory genes in various fish species. In their study they analyzed a stress related heat shock protein in gill tissue of marine and freshwater populations and found that the various genes analysis had not only similarities, but major differences in a higher expression rate of a gene in a saltwater fish compared to freshwater. In our experiment we will be using these similarities and differences to compare how fish who are able to adapt between fresh and saltwater have evolved different or similar protein structures and size according to their environment.
Laudet, V. " Small Changes in Gene Expression of Targeted Osmoregulatory Genes When Exposing Marine and Freshwater to Abrupt Salinity Transfers." PLos ONE. National Center for Biotechnology Information, 2014. Web. 10 Feb. 2017.
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