Research Project Individual Blog Post.
Asmah Tadmori
Our experiment will examine the different types of protein in skeletal muscle tissues among mammals species which they are " Deer, Elk, Caribou, Moose, and Bear", and a chicken leg from birds species to construct a phylogenetic tree that shows the relationship between them.
Normal skeletal muscle tissue can modify their gene expression due to a specific change in the environment, hence, alter its structural composition or the function of the features of its structural components. The basic protein structures of skeletal myocytes that correlated to contraction are myosin and actin filaments. Another protein is Tropomyosin which plays a vital role in controlling when the skeletal muscle should contract, and when it's not. MacLeod has studied three different types of tropomyosin (alpha, betta, gama) in leg chicken, and he compared it to rabbit tropomyosin. He concluded that the amino acid sequence of the tropomyosin of the chicken leg muscle is nearly correlated to the rabbit tropomyosin sequence, and they only vary in 11 positions of the amino acids residues. As we know, rabbits and mammals, and chicken are related to phylum Chordata "vertebrate" which means they might share some traits with each other.
Consequently, the chicken will be distantly related to mammals species so it won't share lost of traits as the members of the mammals species. Checking our gel electrophersis results by comparing the shared proteins bands among these animals will help us determine the relationship among them. Besides, constructing a phylogenetic tree that build on the similarities of amino acids sequences which might provide same synapmorphies but different function.
Resources:
MacLeod, Alexander R. "Distinct α-Tropomyosin mRNA Sequences in Chicken Skeletal Muscle." European Journal Of Biochemistry 126, no. 2 (August 16, 1982): 293-297. Academic Search Premier, EBSCOhost (accessed February 10, 2017).
Link:
http://search.ebscohost.com.edmonds.idm.oclc.org/login.aspx?direct=true&db=aph&AN=15805947&site=ehost-live
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