My group is working on the muscle proteins of six different animals, these are fishes, chickens, pigs, cows, sheep, deer to identify their relationship with each other.
In this research, we use the technique cal gel electrolysis to run samples of proteins and gather the information. Each protein sample should show a specific pattern of band on the gel. We use actin and myosin for one of our standard since every muscle tissue contain a large portion of these. After the gel is run, we will use the information to form a phylogeny tree and proof our hypothesis.
Thus, we set fish as an out group as it doesn't have legs. When we look at chicken we identify it as bipedal while the others are quadruped. In addition, pig is the furthest related to other quadrupeds because cows, sheep and deer are animals that have stomach. In these quadruped animals, deer is the only animal that evolves antlers, so we think that it should be on the newest timeline of the tree.
Reference:
Di Hua HU, Sumiko KIMURA, and Koscak MARUYAMA. 1986. Sodium Dodecyl Sulfate Gel Electrophoresis Studies of
Connectin-Like High Molecular Weight Proteins of
Various Types of Vertebrate and Invertebrate Muscles. Chiba university. 1985 Dec 13 [access 2017 Feb 10].
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