We designed our project to compare muscle protein bands in skeletal muscle and and cardiac muscle from four different organisms. We were afraid that our first gel would be blurry or not run evenly because we loaded samples in all ten lanes but the gel turned out well. Our challenge was related to how long it took us to be measure our bands in lab. The bands had begun to fade and we were concerned that we were unable to measure all of them. We had the intention to run a second gel and measure the bands sooner but our second gel was much blurrier and did not run as evenly as our first gel even though we thought we repeated the process the same way as the first time.
In our error analysis we will likely include that we should have kept the amount of specimen we used from each organism more consistent by weighting them.
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