I
was surprised to see the similarities of muscles in all three specimens. I did
not expect quadrupeds to have such similar proteins with bi-pedals. Our group
also did not have as much smudging in the lanes as we had in the first gel. I
think we held the pipet at an angle when loading the first dye, which the pipet
was held more vertical when loading the second gel. Overall, I saw more similarities
than differences in our gel which, helped me think of advice to give other
groups.
My
advice to other groups is to think of all possibilities. If there was a slight
deviation to the protocol make sure you consider whether that negatively or
positively affected the results you got. Also, another piece of advice is to
make sure at least two people in your group traces the gel. My other lab
partner had a steadier hand which, let them trace in between the proteins more
accurately than I.
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