In our study of vertebrate muscle proteins focusing on
coho salmon (Oncorhynchus kisutch), chicken (Gallus gallus),
pig (Sus scrofa), cow (Bos taurus), deer (Cervidae), and
sheep (Ovis aries), we expect to see more common muscle proteins
between more closely related species. The most abundant proteins in muscle
tissues are actin and myosin, which make up 20% and 60% of the total
myofibrillar proteins, respectively. These contractile proteins are involved in
muscle contraction and many other types of cell movements in vertebrates.
Muscle tissues also include but are not limited to troponin (I, C, T),
tropomyosin, alpha-actinin, and myosin light chain (Obinata et al. 1981). We hypothesized that salmon would be the outgroup
since it is an aquatic organism while other species are terrestrial animals.
In order to examine the relationships among these species, sodium dodecyl sulphate-polyacrylamide gel
electrophoresis (SDS-PAGE) is used to test the hypothesis.
References:
Obinata
T, Maruyama K, Sugita H, Kohama K, Ebashi S. 1981. Dynamic aspects of
structural proteins in vertebrate skeletal muscle. Muscle & Nerve. 4:
456-488. [Internet]. Wiley Online Library; [cited 2017 Feb 09]. Available from:
https://dx.doi.org/10.1002/mus.880040604
No comments:
Post a Comment