Individual Blog Post #2
Asmah Tadmori
The challenge that encountered our group is the presence of some
smearing in our bands that makes it harder for us to detect which is a true
band and which is not. Smearing problem can't help us identify the
relationships between our mammals and chicken easily in our phylogenetic tree.
Another challenge was while pipetting the buffer using a plastic pipette. We
couldn't know if we loaded the appropriate amount of buffer due to the presence
of bubbles during each time of pipetting. My question for other people is that,
Do you guys experience any bubbles while you were pipetting buffer? And Did you
have a smeared bands that made it difficult for you to identify the
similarities between the mammals?
For my error analysis, I would assume that
contamination has occurred especially while we are using the edged razor blade
because we might hold it different ways, and our hands may hold some bacteria.
Specifically while cutting the meats, the meat piece start touching most sides
of the cup, sometimes the edges which the place where hands touch it.
Furthermore, we didn't heat the Actin & Myosin standard at the first gel
running, whereas, in the second gel running, we heated it because we didn't
know if we had to heat it or not. Consequently, I will discuss the contamination
of our skeletal muscle samples, and heating Actin and Ayosin standard sample
consequences. Additionally to the movement of our gel that might lead one of
the samples in the gel to spill out and cause smearing.
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