The only challenge that our group encountered with is that the gels from both trials came out looking smeared. On the first trial, some of our bands came out smeared. We believed this was due to having different sizes of fish muscles from the different organisms.
On the second run, we tried to cut all the fish samples as similar as we could, however some of our bands still came out smeared. In this trial, we definitely had more errors compared to the first trial we ran. One error we encountered with is when we were putting the buffer into the flip tops, it formed bubbles so then when we added the fish muscle, we realized we weren’t able to extract enough protein so we ended up putting more buffer solution into some of the flip tops. This could also explain the smearing on the bands. Another error in this trial is that our fish muscles could have also been different sizes like it was in the first trial. At first we thought of weighing all the fish samples to make sure they were about the same weight before adding the buffer but then we decided against it because we thought that it was unnecessary and it would take up too much time. My question is did any groups weigh the fish muscle before putting it into the buffer solution? If so, did it make a difference weighing it vs not weighing it?
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