While running the samples of mollusc through electrophoresis, our group experienced an issue that delayed our progress. After running the electrophoresis for 30 minutes, we noticed that the gel was nearly torn in half, which forced us to quickly reload the samples for a third time and run it once more. We were fortunate enough to keep the samples with us, which made it possible to run the them again. Another problem in the beginning of the experiment was the mussel and clam were very near each other when we were trying to open them up. It was possible that the two molluscs became contaminated with one another’s proteins, which would affect the results of our gel run.
Our group initially hypothesized that the shrimp would be characterized as the outgroup in our phylogenetic tree, but the results of the bands sort of disagreed with this. When we observed the bands, we noticed that the shrimp actually had very similar bands to the fourth row from the right: scallops. The shrimp was expected to have bands that were drastically different than to any mollusc, but this was not the case. Also, our 5 molluscs were not as similar in terms of bands with each other as expected. The data we found can help us produce a corrected hypothesis that agrees with the comparisons found with the bands.
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