The results that we were most surprised about was that the fishes who live in both salt water and fish water were very different compared to each other since we expected these two fishes to have similar proteins. A challenge we had was picking which types of fish to do the experiment because we wanted fishes that fit our criteria. Originally, we wanted to compare two salt water, two fresh water and one that lives in both but we couldn’t find options for these. We decided to one saltwater fish, one freshwater fish and two fishes that live in both.
We didn’t have many errors while carrying out this lab besides the fact that we basically cooked our fish. During our second run, we forgot to transfer just the buffer with the extracted proteins into a screw cap tube and just put the flip cap tubes into the hot bath. We expected the gels to be smeared due to too many proteins in the gel but the gel had the exact same results as our first one.
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