Friday, February 10, 2017

Research Project Blog #1 (individual)

Nitesh Chetry
Biol& 212 AA
Research Project Blog #1

In our project, my group ran a gel electrophoresis of muscle proteins from a turkey, cougar, bear, pig, sheep, and cow to determine the phylogeny of mammal species, three of them being ungulates, with the turkey as our non-mammalian outgroup.

A simple, observational way to look at the relationship between these five species of mammals may be to look at their food sources and eating patterns. “The clustering by diet (herbivore, omnivore, and carnivore) was highly significant in both the tree-based (Figure S2) and network-based analyses (Figure 1B). In the network-based analysis, host nodes are significantly more connected to other host nodes from the same diet group” (Ley et al. 2008). With more similar food and eating patterns, two species are more likely to be closely related. For example, the tiger is the only carnivore that we chose to observe in lab, likely meaning that it will relate least to the other mammals. In addition, bears and pigs are omnivores which means that they likely are closely related in phylogeny as compared to sheep and cows which are both cud-chewing herbivores. Looking at the eating patterns in these animals gives a reasonable expectation of the phylogeny between these species that may or may not be held up by the data showed by the protein gel electrophoresis.


Ley R, Hamady M, Lozupone C, Turnbaugh P, Ramey R,  Bircher J, Schlegel M, Tucker T, Schrenzel M, Knight R, Gordon J. 2008. Evolution of mammals and their gut microbes. In: National Center for Biotechnology Information [Internet]. Washington, D.C.: American Association for the Advancement of Science; [cited 2017 Feb 10]. Available from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649005/

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