Individual Blog #2

Our mussel sample already died, not much of the mussel was left inside of the shells.

We tried to use the proper part of mussel, but it still did not show the bands as well as the other samples. The first time we ran the gel, too much of the sample was used, and even incorrect muscles for some of our samples. This meant we had to run it one more time. During the second run of the gel electrophoresis, our gel broke into 2 pieces. We re-used the samples that had been sitting outside for more than 30 minutes while the first gel was running.

We predicted the outgroup, shrimp would have a different pattern of bands compared to the other samples. In other words, the shrimp sample should not share bands with any of the other five samples. However, It is hard to say that shrimp is the outgroup from the results of running our gel electrophoresis. We also predicted that only the phylum mollusca would share certain adhesive proteins, with the size being about 500 kDa. We cannot state with full confidence that all the samples except shrimp have similar band size; it seems that the shrimp sample is far too similar. To find a sufficient way to support our hypothesis that shrimp is the outgroup from 6 samples because shrimp belongs in a different phylum, we can state that arthropod, and squid will be the outgroup from phylum molusca(remain 5 samples).