For this resarech project our group will be using Protein Gel Electrophoresis to
compare 5 mammal’s protein (cougar, bear, pig, sheep, cow) and an outgroup (Turkey)
to see if we can find similarities in the structures of protein to link them to
one another phylogenetically. We predicted that since the Cougar is the only carnivorous mammal
that it would branch off first. This would be followed next by the Bear due to
that fact it is omnivous and non domesdicated. Then the pig becaue it is omnivorous
aswell but domesticated. The reason behind putting sheep and cow together at
the end is they are herboviors with a true ruminant disgestive system. True
ruminants, such as cattle, sheep, goats, deer, and antelope, have one stomach
with four compartments: the rumen, reticulum, omasum, and abomasums. (Parish J, Rivera D, Boland H.). This similarity
in digestive tract lead us to believe that similarity in muscle tissue would
arise the closet between these two sets of animal protein when we run the Protein
Gel Eletrophorsis. The group decided to follow Bio Rad Manual that was used in
the fish protein lab only changing the amount of samples that would undergo Protein Gel Electrophoresis from 5 to 6.
Reference:
Dr Jane A. Parish, Dr J. Daniel Rivera Dr Holly T. Boland. Understanding
the Ruminant Animal's Digestive System. The Beef Site. 2009 Aug 22 [accessed 2017 Feb 9] http://www.thebeefsite.com/articles/2095/understanding-the-ruminant-animals-digestive-system/
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